READERS audit

Verdict

Status: keep
Confidence: high
Short verdict: Keep, but avoid implying all RBPs are only post-transcriptional cytoplasmic regulators; many are nuclear and co-transcriptional.

Node definition: RNA-binding proteins that bind RNA sequence/structure/modifications and regulate processing, stability, localization, and translation.
Incoming edges:
- TRIMMER -> READERS: 3’UTR length / → RBP sites
Outgoing edges:
- READERS -> SPLICER: SR/hnRNP / → splice (A) (drawn back/coupled) [context/dashed]
- READERS -> TIMER: HuR (stabilize) / TTP (decay)
- READERS -> FORGE: FMRP represses / initiation (inhibitory)

What is strongly supported: Human cells encode hundreds to over a thousand RBPs with diverse RNA-binding domains; CLIP and perturbation studies show broad control of splicing, decay, localization, and translation.
What is context-dependent: RBP binding is concentration-, compartment-, modification-, and RNA-structure-dependent; motifs are short and often not sufficient for regulation.
What is weak, controversial, or assay-biased: CLIP peaks can be crosslinking/antibody-biased; binding does not guarantee functional effect.
What may be duplicate biology under another name: Overlaps with SPLICER and STAMP readers; SR/hnRNPs appear in multiple nodes.

Missing edges: Add READERS -> TRIMMER and READERS -> VAULT; many RBPs control APA and granule partitioning.
Excess edges: READERS -> FORGE as only FMRP repression is too narrow; RBPs can repress or activate translation.
Candidate splits: No split, but notes could separate splicing RBPs, stability RBPs, translation RBPs.
Candidate merges: No merge.
Candidate renames: RBPs would be clearer.

Concrete graph change, if any: Keep; broaden translation and APA effects and flag CLIP binding-function caveat.
Concrete technical-notes/blog wording change, if any: Mirror the graph recommendation in the glossary and relation catalogue, and explicitly mark the confidence/caveat where the claim is context-dependent or assay-sensitive.